The results show that our method simplifies the controlling logic and realizes the automatic navigation for rat-robots successfully. In navigation experiments, rat-robots learn the controlling methods in short time. Due to the reward-seeking instinct and trial-and-error capability, bio-robot can be steered to keep walking along the right route with rewards and correct its direction spontaneously when rewards are deprived. This paper proposes a new method to realize the automatic navigation for bio-robots with electrical micro-stimulation as real-time rewards. Current automatic navigation methods for bio-robots focus on the controlling rules to force animals to obey man-made commands, with animals' intelligence ignored. Sun, Chao Zhang, Xinlu Zheng, Nenggan Chen, Weidong Zheng, Xiaoxiangīio-robots that controlled by outer stimulation through brain computer interface (BCI) suffer from the dependence on realtime guidance of human operators. This evaluation demonstrates that the QIAGEN EZ 1 DSP Virus Kit provides an attractive solution for fully automated, low throughput sample preparation for use with the Abbott RealTime HIV- 1, HCV, and HBV assays.īio-robots automatic navigation with electrical reward stimulation. In addition, HCV and HBV viral load measurements after BioRobot EZ 1 DSP extraction correlated well with those obtained after Abbott m2000sp extraction.
No cross-contamination was observed, as well as no negative impact of elevated levels of various interfering substances. Excellent precision was obtained (inter-assay standard deviation for HIV- 1: 0.06-0.17 log copies/ml (>2.17 log copies/ml), HCV: 0.05-0.11 log IU/ml (>2.09 log IU/ml), HBV: 0.03-0.07 log copies/ml (>2.55 log copies/ml)), with good sensitivity (95% hit rates for HIV- 1: 50 copies/ml, HCV: 12.5 IU/ml, HBV: 10 IU/ml).
Linearity was observed within the tested ranges (for HIV- 1: 2.0-6.0 log copies/ml, HCV: 1.3-6.9 log IU/ml, HBV: 1.6-7.6 log copies/ml). The following performance characteristics were evaluated: linear range and precision, sensitivity, cross-contamination, effects of interfering substances and correlation. In this study, the performance of QIAGEN EZ 1 DSP Virus Kit on the BioRobot EZ 1 DSP was evaluated in combination with the Abbott RealTime HIV- 1, HCV, and HBV assays, followed by thermalcycling and detection on the Abbott m2000rt platform. Schneider, George J Kuper, Kevin G Abravaya, Klara Mullen, Carolyn R Schmidt, Marion Bunse-Grassmann, Astrid Sprenger-Haussels, MarkusĪutomated sample preparation systems must meet the demands of routine diagnostics laboratories with regard to performance characteristics and compatibility with downstream assays. Performance evaluation of the QIAGEN EZ 1 DSP Virus Kit with Abbott RealTime HIV- 1, HBV and HCV assays. of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs. In conclusion, we have demonstrated that extraction of DNA from various types.We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ 1 Workstation. Of 298 samples extracted, 11 (4%) did not yield acceptable results. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Stangegaard, Michael Jørgensen, Mads Hansen, Anders Johannes
Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ 1 Workstation
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